Platelet Lysate-Modified Porous Silicon Microparticles for Enhanced Cell Proliferation in Wound Healing Applications

The new frontier in the treatment of chronic
nonhealing wounds is the use of micro-and nanoparticles to
deliver drugs or growth factors into the wound. Here, we used
platelet lysate (PL), a hemoderivative of platelets, consisting of
a multifactorial cocktail of growth factors, to modify porous
silicon (PSi) microparticles and assessed both in vitro and ex
vivo the properties of the developed microsystem. PL-modified
PSi was assessed for its potential to induce the proliferation of
fibroblasts. The wound closure-promoting properties of the
microsystem were then assessed in an in vitro wound-healing
assay. Finally, the PL-modified PSi microparticles were
evaluated in an ex vivo experiment over human skin. It was
shown that PL-modified PSi microparticles were cytocompatible and enhanced cell proliferation in different
experimental settings. In addition, this microsystem promoted
the closure of the gap between the fibroblast cells in the
wound healing assay, in periods compared with the
positive control, and induced a proliferation and regeneration
process onto the human skin in an ex vivo experiment. Overall,
our results show that PL-modified PSi microparticles are suitable microsystems for further development toward applications in
the treatment of chronic nonhealing wounds.