Two-photon excitated fluorescence microscope

The microscope (100) has a laser source (1) suitable for emitting a light beam. An optical arrangement (3,5,6) receives light beam from the laser source and shapes the light beam so that the light beam is collimated in a first transverse direction (x) perpendicular to a propagation direction (z) of the light beam at an output of microscope. The light beam is focused in a second transverse direction (y) perpendicular to the first transverse direction and to propagation direction to form a line parallel to the first transverse direction.