Steady-state and pulse-chase analyses of fibrillar collagen

Capitolo di libro

Data di Pubblicazione:

2019

Abstract:

Steady-state and pulse-chase collagen analyses are powerful approaches to investigate in vitro the structure of collagen and its kinetic of secretion, respectively. The electrophoretic analysis of purified 3H-proline-labeled collagen allows to determine the nature and post translational modifications of its α chains, whereas short-pulse labeling can be used to follow collagen secretion over time.

Tipologia CRIS:

2.1 Contributo in volume (Capitolo o Saggio)

Keywords:

Collagen; Osteogenesis imperfecta; Post translational modifications; Pulse-chase; Steady-state; Animals; Electrophoresis, Polyacrylamide Gel; Fibrillar Collagens; Fibroblasts; Humans; Proline; Protein Processing, Post-Translational; Tritium; Urea

Elenco autori: