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HPV Infection Affects Human Sperm Functionality by Inhibition of Aquaporin-8

Articolo
Data di Pubblicazione:
2020
Abstract:
Human sperm cells express different aquaporins (AQPs), AQP3, 7, 8, 11, which are localized both in the plasma membrane and in intracellular structures. Besides cell volume regulation and end stage of cytoplasm removal during sperm maturation, the role of AQPs extends also to reactive oxygen species (ROS) elimination. Moreover, oxidative stress has been shown to inhibit AQP-mediated H2O2 permeability. A decrease in AQPs functionality is related to a decrease in sperm cells number and motility. Here we investigate the possible effect of human Papillomavirus (HPV) on both expression and function of AQPs in human sperm cells of patients undergoing infertility couple evaluation. Stopped-flow light-scattering experiments demonstrated that HPV infection heavily reduced water permeability of sperm cells in normospermic samples. Confocal immunofluorescence experiments showed a colocalization of HPV L1 protein with AQP8 (Pearson's correlation coefficient of 0.61), confirmed by co-immunoprecipitation experiments. No interaction of HPV with AQP3 and AQP7 was observed. A 3D model simulation of L1 protein and AQP8 interaction was also performed. Present findings may suggest that HPV infection directly inhibits AQP8 functionality and probably makes sperm cells more sensitive to oxidative stress.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
aquaporin; oxidative stress; sperm motility; sterility; water channel
Elenco autori:
Pellavio, Giorgia; Todaro, Federica; Alberizzi, Paola; Scotti, Claudia; Gastaldi, Giulia; Lolicato, Marco; Omes, Claudia; Caliogna, Laura; Nappi, Rossella E; Laforenza, Umberto
Autori di Ateneo:
LAFORENZA UMBERTO
LOLICATO MARCO GAETANO
NAPPI ROSSELLA
OMES CLAUDIA
PELLAVIO GIORGIA
SCOTTI CLAUDIA
Link alla scheda completa:
https://iris.unipv.it/handle/11571/1340731
Link al Full Text:
https://iris.unipv.it//retrieve/handle/11571/1340731/356825/cells-09-01241.pdf
Pubblicato in:
CELLS
Journal
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