Data di Pubblicazione:
2004
Abstract:
In this work, a new type of penicillin G acylase (PGA)-based monolithic silica support was developed and evaluated for the chiral
separation in HPLC. The preparation procedure consisted of two steps: preparation of an epoxy derivatized monolithic silica column and
chemical modification of the epoxide groups with the enzyme chiral selector. The epoxy Silica-Rod column for the immobilization of PGA
was prepared with the in situ modification process by using epoxy-silanes and the identification of the species bound to the surface was
achieved by solid-state nuclear magnetic resonance. The enzyme was covalently immobilized to the surface of the derivatized monolithic
column. The enantioselectivity and the performance of the developed column are discussed and compared to the corresponding experimental
data obtained with a PGA-based microparticulate (u5m) silica column.
separation in HPLC. The preparation procedure consisted of two steps: preparation of an epoxy derivatized monolithic silica column and
chemical modification of the epoxide groups with the enzyme chiral selector. The epoxy Silica-Rod column for the immobilization of PGA
was prepared with the in situ modification process by using epoxy-silanes and the identification of the species bound to the surface was
achieved by solid-state nuclear magnetic resonance. The enzyme was covalently immobilized to the surface of the derivatized monolithic
column. The enantioselectivity and the performance of the developed column are discussed and compared to the corresponding experimental
data obtained with a PGA-based microparticulate (u5m) silica column.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
Chiral Stationary Phases; LC; Monolithic Columns
Elenco autori:
Calleri, Enrica; Massolini, Gabriella; Lubda, D.; Temporini, Caterina; Loiodice, F.; Caccialanza, Gabriele
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