Data di Pubblicazione:
2008
Abstract:
ABSTRACT: Photodynamic treatment (PDT) has been proposed as a new approach for inactivation
of biofilms associated with medical devices that are resistant to chemical additives or biocides. In
this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing
dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus
epidermidis biofilms. Effect of the combined photodynamic action of MC 540 and 532 nm
laser was investigated on the viability and structure of biofilms of two Staphylococcus epidermidis
strains, RP62A and 1457. Significant inactivation of cells was observed when biofilms were exposed
to MC 540 and laser simultaneously. The effect was found to be light dose-dependent but S. epidermidis
1457 biofilm proved to be slightly more susceptible than S. epidermidis RP62A biofilm. Furthermore,
significant killing of both types of cells was attained even when a fixed light dose was delivered
to the biofilms. Confocal laser scanning microscope (CLSM) analysis indicated damage to
bacterial cell membranes in photodynamically treated biofilms, while disruption of PDT-treated
biofilm was confirmed by scanning electron microscopy (SEM).
of biofilms associated with medical devices that are resistant to chemical additives or biocides. In
this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing
dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus
epidermidis biofilms. Effect of the combined photodynamic action of MC 540 and 532 nm
laser was investigated on the viability and structure of biofilms of two Staphylococcus epidermidis
strains, RP62A and 1457. Significant inactivation of cells was observed when biofilms were exposed
to MC 540 and laser simultaneously. The effect was found to be light dose-dependent but S. epidermidis
1457 biofilm proved to be slightly more susceptible than S. epidermidis RP62A biofilm. Furthermore,
significant killing of both types of cells was attained even when a fixed light dose was delivered
to the biofilms. Confocal laser scanning microscope (CLSM) analysis indicated damage to
bacterial cell membranes in photodynamically treated biofilms, while disruption of PDT-treated
biofilm was confirmed by scanning electron microscopy (SEM).
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
TERAPIA FOTODINAMICA (PDT); Merocianina 540 (MC 540); S. EPIDERMIDIS BIOFILMS
Elenco autori:
Sbarra, MARIA SONIA; DI POTO, Antonella; Arciola, C. R.; Saino, Enrica; Sharma, Mrinalini; Bragheri, Francesca; Cristiani, Ilaria; Speziale, Pietro; Visai, Livia
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