Properties of Bacillus subtilis mutants temperature sensitive in germination

A new mutant of Bacillus subtilis defective in the outgrowth phase of spore germination has been isolated. When incubated at 46 C, the spores of the mutant gave rise to abnormally large swollen cells. Genetic crosses show that the mutant is different from the three previously described. The genetic analysis indicates two regions of the B. subtilis chromosome involved in the control of the spore outgrowth.