Data di Pubblicazione:
2021
Abstract:
Abstract: The placental methylation pattern is crucial for the regulation of genes involved in trophoblast invasion and placental development, both key events for fetal growth. We investigated
LINE-1 methylation and methylome profiling using a methylation EPIC array and the targeted
methylation sequencing of 154 normal, full-term pregnancies, stratified by birth weight percentiles.
LINE-1 methylation showed evidence of a more pronounced hypomethylation in small neonates
compared with normal and large for gestational age. Genome-wide methylation, performed in two
subsets of pregnancies, showed very similar methylation profiles among cord blood samples while
placentae from different pregnancies appeared very variable. A unique methylation profile emerged
in each placenta, which could represent the sum of adjustments that the placenta made during the
pregnancy to preserve the epigenetic homeostasis of the fetus. Investigations into the 1000 most
variable sites between cord blood and the placenta showed that promoters and gene bodies that
are hypermethylated in the placenta are associated with blood-specific functions, whereas those
that are hypomethylated belong mainly to pathways involved in cancer. These features support the
functional analogies between a placenta and cancer. Our results, which provide a comprehensive
analysis of DNA methylation profiling in the human placenta, suggest that its peculiar dynamicity
can be relevant for understanding placental plasticity in response to the environment.
Tipologia CRIS:
1.1 Articolo in rivista
Elenco autori:
Rondinone, Ornella; Murgia, Alessio; Costanza, Jole; Tabano, Silvia; Camanni, Margherita; Corsaro, Luigi; Fontana, Laura; Colapietro, Patrizia; Calzari, Luciano; Motta, Silvia; Santaniello, Carlo; Radaelli, Tatjana; Ferrazzi, Enrico; Bosari, Silvano; Gentilini, Davide; Sirchia, Silvia; Miozzo, Monica
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