Data di Pubblicazione:
2012
Abstract:
Microtubules (MTs) control cell replication, material transport and motion in eukaryotic cells, but MT role in several pathologies is still unknown. These functions are related to the MT physico-chemical properties and MT formation mode starting from tubulin molecules. This study describes a new method, based on
the computer aided analysis of the electron paramagnetic resonance (EPR) spectra of selected spin probes to obtain structural and dynamical information on tubulins and MTs and the kinetics of MTs formation promoted by guanosine-50-triphosphate (GTP). It was found that tubulin and MTs avoid radical quenching
caused by ethylene glycol tetraacetic acid (EGTA). MT formation showed different kinetics as a function of tubulin concentration. At 5 mg/mL of tubulin, MTs were formed in 8 min. These results are also useful for getting information on MT–drug interactions.
the computer aided analysis of the electron paramagnetic resonance (EPR) spectra of selected spin probes to obtain structural and dynamical information on tubulins and MTs and the kinetics of MTs formation promoted by guanosine-50-triphosphate (GTP). It was found that tubulin and MTs avoid radical quenching
caused by ethylene glycol tetraacetic acid (EGTA). MT formation showed different kinetics as a function of tubulin concentration. At 5 mg/mL of tubulin, MTs were formed in 8 min. These results are also useful for getting information on MT–drug interactions.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
EPR; Tubulin; Microtubules; Nitroxide; GTP
Elenco autori:
Ottaviani, M. F.; Pregnolato, Massimo; Cangiotti, M.; Fiorani, L.; Fattori, A.; Danani, A.
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