ID:
502139
Duration (hours):
72
CFU:
9
SSD:
CHIMICA ANALITICA
Year:
2025
Overview
Date/time interval
Primo Semestre (22/09/2025 - 16/01/2026)
Syllabus
Course Objectives
The aim of the course is to give the student the fundamental concepts relative to sample preparation so that the analytes to be determined are in the chemical form useful for the instrumental analysis that will follow; to critically illustrate the principles of the instrumental techniques (atomic and molecular spectroscopy, mass spectrometry and hyphenated techniques) in order to provide the student with the necessary knowledge for a critical choice of the most suitable technique for solving the various problems that arise and to be able to assess the performance, criticality and interferences of each technique. Of the various techniques the student will know the pros and cons in order to be able to make, when possible, reasoned choices in terms of accuracy of the required analytical result, cost, time, safety and environmental sustainability.
Course Prerequisites
The student is required to have an adequate initial grounding in the fundamental principles of chemistry covered in the basic courses (general and inorganic chemistry and stoichiometry, chemistry-physics, analytical and organic chemistry) and in the basic notions of mathematics indispensable to be able to critically learn the instrumental analysis techniques explained in the course, with particular reference to concepts relating to molecules and atoms, ions and excited species, absorption and emission, chemical equilibrium, pH and species distribution, basic calculations, logarithms, derivatives.
Teaching Methods
Lectures are given with the aid of slides (made available to the student) on which to follow the content. The explanations will be repeated in front of the main instruments present in analytical laboratories, so that the student can see the characteristic parts of the equipment and the set-up for performing an analysis. In the classroom, exercises will be carried out on calculating concentrations in real samples and in different matrices involving preconcentration, cleanup, dilution, etc., in order to apply the concepts seen in theory and facilitate learning.
Assessment Methods
The examination takes place in oral form, on the topics of the course, with references to cases seen together in the classroom (in any case available on the slides made available) and with a request to set up problem-solving related to the first part of the course (sample treatment and concentration calculations) in order to test the student's knowledge of the topics covered in the course and his/her ability to correctly apply the various information learned to the analysis of real samples.
Texts
The same book is also available in English “Principles of Instrumental Analysis”, D.A. Holler, F.J. Skoog, S.R. Crouch, Skoog- Leary- International Edition.
Contents
Part of the course will be devoted to sample handling according to the matrix, the analytes to be determined and their concentration, and how to work to avoid contamination (laboratory glassaware, reagents, laboratory equipment,...), which is all the more important the more sensitive the analytical methods are. The principles of the Green Analytical Chemistry (GAC) will be illustrated in terms of reduction of reagents, solvents, energy consumption, waste, attention to operator safety; signal/noise ratio, its importance in the treatment of analytical data, detection and quantification limits and their significance; the main sources of noise and how to minimise it. This is followed by lectures on quantitative analysis, where the main calculation methods (straight line with external standard, standard addition method, internal standard) are compared, the differences, the criteria of choice, accompanied by calculation examples and a critical evaluation of the results. The course then explains the main spectroscopic techniques used in Analytical Chemistry (more briefly those now less frequently used such as Atomic Emission in Flame, Atomic Absorption in Flame and in Graphite Furnace and then in depth atomic emission in plasma with optical detector; in-depth UV-Vis spectrophotometry; fluorescence, molecular phosphorescence and chemiluminescence and comparison with UV-Vis molecular spectroscopy); Mass spectrometry and hyphenated techniques (GC-MS, HPLC-MS; ICP-MS). For each of the techniques, the principles, instrumentation and some applications are presented. Some lectures of the course will be devoted to the main analytical techniques for surface characterisation. At the end of the course the student will be able to evaluate the advantages and criticalities of the different analytical techniques and to choose, when possible, the best solution in terms of analytical results, cost, time, safety and environmental sustainability.
Course Language
Italian
More information
Class attendance is recommended.
Further information can be requested by e-mail
Further information can be requested by e-mail
Degrees
Degrees
Chemistry
Bachelor’s Degree
3 years
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