ID:
502047
Duration (hours):
144
CFU:
12
SSD:
BIOLOGIA APPLICATA
Year:
2025
Overview
Date/time interval
Annualità Singola (24/09/2025 - 12/06/2026)
Syllabus
Course Objectives
Clinical Biochemistry: acquisition of theoretical and practical knowledge related to the main techniques used in clinical laboratories. Modern approaches of biomedical research will be presented with the aim to make the student aware of the scientific path necessary to investigate and understand the biochemical basis of human pathologies and to identify new disease biomarkers.
Histology Laboratory: acquisition by the student of theorical and practical knowledges related to the main morphological investigation methods to understand and describe the structural organization of animal tissues through histological observations of healthy preparations.
Therefore, at the end of the histo lab, the student will:
- know how to use a common transmitted light optical microscope;
- know the main methods and tools of the morphological investigation by the microscope (having understood the basics of some analytical procedures to determine the structure-function relationship of cells in tissues and tissues within the organ)
- being able to describe a common histological slide, evaluating the morphological characteristics of cells and tissues, and their structural organization, also thanks to the different dye affinities with the histological dyes used.
Microbiology Laboratory: Acquisition by the students of knowledge related to the diagnostic microbiology principles and the laboratory role in monitoring and preventing antibiotic resistance.
Genetics Laboratory.
The laboratory activity is aimed at showing the diagnostic process to be undertaken in patients in whom a genetic disease is clinically suspected. The activities that will be carried out will be mainly based on learning the main molecular and bioinformatics techniques used in a medical genetics laboratory.Laboratory activity will provide the opportunity to experience first-hand the activity carried out by a modern biotechnologist in a diagnostic/research context.
Histology Laboratory: acquisition by the student of theorical and practical knowledges related to the main morphological investigation methods to understand and describe the structural organization of animal tissues through histological observations of healthy preparations.
Therefore, at the end of the histo lab, the student will:
- know how to use a common transmitted light optical microscope;
- know the main methods and tools of the morphological investigation by the microscope (having understood the basics of some analytical procedures to determine the structure-function relationship of cells in tissues and tissues within the organ)
- being able to describe a common histological slide, evaluating the morphological characteristics of cells and tissues, and their structural organization, also thanks to the different dye affinities with the histological dyes used.
Microbiology Laboratory: Acquisition by the students of knowledge related to the diagnostic microbiology principles and the laboratory role in monitoring and preventing antibiotic resistance.
Genetics Laboratory.
The laboratory activity is aimed at showing the diagnostic process to be undertaken in patients in whom a genetic disease is clinically suspected. The activities that will be carried out will be mainly based on learning the main molecular and bioinformatics techniques used in a medical genetics laboratory.Laboratory activity will provide the opportunity to experience first-hand the activity carried out by a modern biotechnologist in a diagnostic/research context.
Course Prerequisites
Clinical Biochemistry: basic knowledge of Biochemistry and Clinical Biochemistry.
Histology Laboratory: basic elements of Cell Biology, Chemistry and Biochemistry.
Microbiology Laboratory:
Basic knowledge of Microbiology, referring in particular to the structure of the bacterial cell and antibiotics.
Genetics Laboratory. Good knowledge in the field of general and molecular biology and human genetics: mitosis and meiosis; structure and function of DNA and RNA; protein synthesis; genes and chromosomes; Mendel's laws of Inheritance; patterns of inheritance (dominant/recessive/x-linked); genetic mutations and pathogenetic mechanisms associated with diseases (GoF, LoF, dominant-negative).
Histology Laboratory: basic elements of Cell Biology, Chemistry and Biochemistry.
Microbiology Laboratory:
Basic knowledge of Microbiology, referring in particular to the structure of the bacterial cell and antibiotics.
Genetics Laboratory. Good knowledge in the field of general and molecular biology and human genetics: mitosis and meiosis; structure and function of DNA and RNA; protein synthesis; genes and chromosomes; Mendel's laws of Inheritance; patterns of inheritance (dominant/recessive/x-linked); genetic mutations and pathogenetic mechanisms associated with diseases (GoF, LoF, dominant-negative).
Teaching Methods
Clinical Biochemistry: calculations and preparation of reagents, practical activities at the bench, analysis, and discussion of results. Each student has the opportunity to carry out practical tasks with the assistance of the Professors and tutors.
Practical Histo labs: individual observations at the optical microscope of healthy histological slides and probably comparative descriptions with pathological tissues.
Microbiology Laboratory: laboratory exercises.
Genetics Laboratory. Hands-on sessions in which students will be directly engaged in a molecular laboratory context. In addition, lessons aimed at the analysis and interpretation of NGS data will be held in the computer classrooms of the university.
Practical Histo labs: individual observations at the optical microscope of healthy histological slides and probably comparative descriptions with pathological tissues.
Microbiology Laboratory: laboratory exercises.
Genetics Laboratory. Hands-on sessions in which students will be directly engaged in a molecular laboratory context. In addition, lessons aimed at the analysis and interpretation of NGS data will be held in the computer classrooms of the university.
Assessment Methods
Clinical Biochemistry: during the practice, students are required to fill out technical sheets that are useful for setting up experiments (e.g., calculating reagent weights, volumes, dilutions, etc.). it is mandatory to attend at least the 75% of the lab hours. Evaluation scale: pass/fail grading.
Histology Laboratory: written exam at the end of the lab.
Genetics Laboratory. Attendance of lab activities: it is mandatory to attend at least the 75% of the lab hours. Evaluation scale: pass/fail grading.
Histology Laboratory: written exam at the end of the lab.
Genetics Laboratory. Attendance of lab activities: it is mandatory to attend at least the 75% of the lab hours. Evaluation scale: pass/fail grading.
Texts
Clinical Biochemistry: materials provided by the Professors.
Histology Laboratory: tests and Atlas, as:
-Wheater, Istologia e anatomia microscopica
- Ross, Atlante di Istologia e Anatomia microscopica
- Stevens, Istologia Umana.
Microbiology Laboratory: materials provided by the teacher.
Genetics Laboratory: materials provided by the teacher.
Histology Laboratory: tests and Atlas, as:
-Wheater, Istologia e anatomia microscopica
- Ross, Atlante di Istologia e Anatomia microscopica
- Stevens, Istologia Umana.
Microbiology Laboratory: materials provided by the teacher.
Genetics Laboratory: materials provided by the teacher.
Contents
Clinical biochemistry: the activity consists of practical activities of small working-groups to allow all students working involved in every experimental phase and setup: preparation, execution, and analysis. Main methodologies covered will include: cell isolation and counting, preparation of protein lysates, protein electrophoresis and Western blotting, ELISA assays, sample preparation for immunofluorescence, microscopy, and image analysis.
Histology Laboratory:
-Methods and tools for morphological investigations: the optical microscope as the tool of choice for histological analysis.
-Preparation of a tissue slide: fixation, inclusion, cutting, staining
- Practical example of a histological staining
Microbiology Laboratory: pratical exercises concerning to the
cultivation of microorganisms in culture media,
Gram-staining and microscopic examination,
biochemical-metabolic test for microorganisms identification,
antibiotic susceptibility test by Kirby-Bauer and determination of Minimum Inhibitory Concentration (M.I.C.) by E-test.
Reading and interpretation of antibiotic susceptibility testing results.
Experiment of enzymatic extraction and of bacteria count.
Test to ESBL and carbapenemases identification.
Genetics Laboratory. Students will be personally involved in achieving a molecular diagnosis in real clinical cases by applying the main molecular techniques used in a medical genetics laboratory, such as: DNA extraction, PCR amplification, gel electrophoresis and subsequent Sanger sequencing.In addition, lessons aimed at the analysis and interpretation of NGS data will be held in the computer classrooms of the university.
Histology Laboratory:
-Methods and tools for morphological investigations: the optical microscope as the tool of choice for histological analysis.
-Preparation of a tissue slide: fixation, inclusion, cutting, staining
- Practical example of a histological staining
Microbiology Laboratory: pratical exercises concerning to the
cultivation of microorganisms in culture media,
Gram-staining and microscopic examination,
biochemical-metabolic test for microorganisms identification,
antibiotic susceptibility test by Kirby-Bauer and determination of Minimum Inhibitory Concentration (M.I.C.) by E-test.
Reading and interpretation of antibiotic susceptibility testing results.
Experiment of enzymatic extraction and of bacteria count.
Test to ESBL and carbapenemases identification.
Genetics Laboratory. Students will be personally involved in achieving a molecular diagnosis in real clinical cases by applying the main molecular techniques used in a medical genetics laboratory, such as: DNA extraction, PCR amplification, gel electrophoresis and subsequent Sanger sequencing.In addition, lessons aimed at the analysis and interpretation of NGS data will be held in the computer classrooms of the university.
Course Language
Italian
More information
Clinical Biochemistry contacts:
alessandra.balduini@unipv.it
christian.dibuduo@unipv.it
Reception for Histo Lab: by appointment, after contacting the teacher by e-mail at:
federica.riva01@unipv.it
For Genetics:
elisa.giorgio@unipv.it
paola.dimartino@unipv.it
Microbiology Laboratory:
irene.cassaniti01@universitadipavia.it
alessandra.balduini@unipv.it
christian.dibuduo@unipv.it
Reception for Histo Lab: by appointment, after contacting the teacher by e-mail at:
federica.riva01@unipv.it
For Genetics:
elisa.giorgio@unipv.it
paola.dimartino@unipv.it
Microbiology Laboratory:
irene.cassaniti01@universitadipavia.it
Degrees
Degrees
BIOTECHNOLOGY
Bachelor’s Degree
3 years
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People
People (6)
Teaching staff
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