ID:
508318
Duration (hours):
48
CFU:
6
SSD:
GENETICA
Year:
2025
Overview
Date/time interval
Secondo Semestre (02/03/2026 - 12/06/2026)
Syllabus
Course Objectives
The course presents a detailed overview of the recombinant DNA technology from the the cloning and production of recombinant proteins in bacterial and eukaryotic systems to the analysis of novel and emerging techniques, such as genome editing. Examples of the application of recombinant DNA technology will be presented in the fields of research, medicine, agriculture and livestock breeding.
At the end of the course students 1) will have the basic knowledge of recombinant DNA technologies, including genome editing; 2) will be able to apply the acquired knowledge to planning and performing basic experiments involving recombinant DNA; 3) will be able to evaluate knowingly and discuss the growing impact that recombinant DNA technology is having on multiple aspects of our social life.
At the end of the course students 1) will have the basic knowledge of recombinant DNA technologies, including genome editing; 2) will be able to apply the acquired knowledge to planning and performing basic experiments involving recombinant DNA; 3) will be able to evaluate knowingly and discuss the growing impact that recombinant DNA technology is having on multiple aspects of our social life.
Course Prerequisites
Given the topics covered by the course it is mandatory to have knowledge of Molecular Biology, Biochemistry, Genetics and Chemistry.
Teaching Methods
Classes last 48 hours and are mainly lessons with the projection and comment of slides and visualization of videos and animations from specialized web sites. Given the rapid changes in recombinant DNA technology scientific articles and reviews might be used to have an updated picture of selected topics. All the slides and materials used in classes will be published on the course web site through Kiro, the e-learning platform of the University of Pavia.
At the end of the course the final exam is discussed with students and an in-class simulation is performed. The simulation is then posted on the course web site so that students can keep practicing on it.
At the end of the course the final exam is discussed with students and an in-class simulation is performed. The simulation is then posted on the course web site so that students can keep practicing on it.
Assessment Methods
There will be neither home assignments nor tests during the classes period. There will be only one final exam. It is sustained on the university Kiro-Testing platform. It is made of open and multiple-choice questions (typically 20) based on the arguments covered by the course.
Texts
The course reference textbook is Gene Cloning and DNA Analysis: an Introduction by T.A. Brown, 7th ed. Wiley and Sons, 2016. Some arguments will be taken from “Molecular Biotechnology” by B.R. Glick e C.L. Patten, 7th editionASM Press 2017, but in that case following the lessons and studying the materials posted on the course web site on the e-learning platform Kiro, will be enough for the final exam.
A useful textbook, more oriented on the technical aspects of recombinant DNA procedures is Tecniche e Metodi per la Biologia Molecolare byi Francesco Amaldi, Piero Benedetti, Graziano Pesole, Paolo Plevani, 2020 CEA (in italian)
A useful textbook, more oriented on the technical aspects of recombinant DNA procedures is Tecniche e Metodi per la Biologia Molecolare byi Francesco Amaldi, Piero Benedetti, Graziano Pesole, Paolo Plevani, 2020 CEA (in italian)
Contents
The isolation and analysis of nucleic acids. The enzymes of recombinant DNA technology. The molecular cloning concept. Cloning vectors for prokaryotes and eukaryotes. PCR and cloning. Real Time PCR. DNA libraries: types, construction and uses. The selection of cloned DNA. Prokaryotic and eukaryotic host/vector systems for the overproduction of recombinant proteins. DNA mutagenesis and protein engineering. Application of recombinant DNA in medicine, agricolture, livestock. Genome editing: ZFN, TALEN, CRISPR-Cas. Archeogenomics.
Course Language
Italian
More information
Contact information: Prof. Giulia Barbieri, Department of Biology and Biotechnology, via Ferrata 9, Pavia. E-mail: giulia.barbieri@unipv.it; Tel. 0382 985571. For an appointment contact via E-mail.
The course has a dedicated web site on the e-learning portal of the University of Pavia, Kiro, that students can access using their login credentials.
For the categories of students identified by the Academic Senate in the session of March 23rd 2023, inclusive teaching methods will be implemented and provided on an ad hoc basis.
The course has a dedicated web site on the e-learning portal of the University of Pavia, Kiro, that students can access using their login credentials.
For the categories of students identified by the Academic Senate in the session of March 23rd 2023, inclusive teaching methods will be implemented and provided on an ad hoc basis.
Degrees
Degrees
BIOTECHNOLOGY
Bachelor’s Degree
3 years
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