Paternal origin of the X chromosome in a 63,X Italian Trotter mare

In this work, we report information about a new case of a 63,X chromosome constitution in a mare and we established, for the first time in this specie, the parental origin of the only X chromosome present.The cytogenetic analysis of 100 metaphases, using both Giemsa staining, CBG banding and FISH technique, shown a 2n=63,X condition with no sign of mosaicism, while paternal origin of the only X chromosome present was ascertained analysing two high informative microsatellites: LEX024 and LEX003.