Publication Date:
2018
abstract:
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.
Iris type:
2.1 Contributo in volume (Capitolo o Saggio)
Keywords:
3D culture; Alginate; Encapsulation; Granulosa cells
List of contributors:
Faustini, Massimo; Curone, Giulio; Torre, Maria L; Vigo, Daniele
Book title:
Epithelial Cell Culture. Methods in Molecular Biology