Publication Date:
1987
abstract:
A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.
Iris type:
1.1 Articolo in rivista
List of contributors:
Morelli, L; Cocconcelli, Ps; Bottazzi, V; Damiani, G; Ferretti, Luca; Sgaramella, V.
Published in: