Publication Date:
1998
abstract:
The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase.
Iris type:
1.1 Articolo in rivista
Keywords:
MTCY428.08; Mycobacterium tuberculosis; NAD synthetase
List of contributors:
Cantoni, RITA MARIA; Branzoni, Manuela; Labo', M.; Rizzi, M.; Riccardi, Giovanna
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