Immunomagnetic separation and biological characterization of a putative mesenchymal stem cells subpopulation

Our previous data demonstrated the presence of MSCs isolated from ovarian follicular liquid. A novel immunomagnetic procedure to isolate rere cells in suspension was used, using Dynal microbeads and a dedicated multiwells magnetic device. It was possible to isolate CD44+ cells and recovere them onto coverslips for the next steps of immunostaining and/or biological characterization. Further experiments have been designed in order to verify the stemness of these cells, seeding them in culture and inducing differentiation into other cell lineages to assess in vitro their plasticity.