Assessment of DNA Damage by Micellar Electrokinetic ChromatographyCapillary Electrophoresis of Biomolecules
Chapter
Publication Date:
2013
abstract:
A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of uncanonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR templates used in several fields of investigation.
Iris type:
2.1 Contributo in volume (Capitolo o Saggio)
List of contributors:
Fattorini, P.; MARRUBINI BOULAND, GIORGIO CARLO; Grignani, Pierangela; Sorçaburu Cigliero, S.; Previdere', Carlo
Book title:
Methods in Molecular Biology Capillary Electrophoresis of Biomolecules
Published in: