Isolation of high molecular weight components and contribution to the protective activity of coffee against lipid peroxidation in a rat liver microsome system.

One of the most extensively studied and best-established properties of coffee is its antioxidant activity.
We have shown that coffee brew has the ability to inhibit lipid peroxidation completely in a rat liver
microsome biological system. The inhibitory activity was mainly due to the high molecular weight
(HMW) fraction; this consisted of five components that were isolated, purified, and seen to occur in
different amounts in the brew. Each component had different spectra and element compositions,
although they all contained nitrogen. HMW, nitrogen content, and brown color enabled three
components to be attributed to the melanoidin family; the two nonbrown components could not be
considered as melanoidins. Each melanoidin and nonmelanoidin component contributes to a different
extent to the protective action exerted by coffee brew. None of the isolated components completely
inhibited microsomal lipid peroxidation alone, suggesting that each acts at different sites and/or
possesses different mechanisms of action. The protective activity of coffee brew is thus underpinned
by the antiradical properties, reducing power, and metal chelating ability of the individual components,
each contributing to a different extent.