1) Know the techniques of fixation and inclusion of histological samples and the problems corelated to the pre-analytical phase. 2) Know the technical principles underlying the main histochemical stains used in pathological anatomy 3) Know the main practical applications of histochemical staining. 4) Know and describe the main immunohistochemical staining techniques 5) Describe the main practical applications of immunohistochemistry in diagnostic pathology.
Course Prerequisites
Basic knowledge of the principles of general chemistry, anatomy and biology
Teaching Methods
Lectures and presentation of practical application of the different histochemical and immunohistochemical techniques. Professor is available for clarification on the topics of the program.
Assessment Methods
Oral exam with multiple questions aimed at ascertaining the knowledge acquired on the main histologic, histochemical and immunohistochemical techniques and their practical application. The assessment will also take into account the ability to communicate and use an appropriate scientific language.
Texts
Robbins, Pathological Bases of Diseases.
Contents
1) Histological samples fixation and paraffin embedding techniques in surgical pathology lab 2) Routinary histological stainings 3) Special histochemical stainings (Pas, Alcina blue, PAS-Alcian, Congi Red etc): technical principles and practical applications in surgical pathology 4) Immunohistochemistry: history and general principles 5) Primary antibodies and antisera 6) Immunohistochemical stainings detection methods and signal enhancement techniques 7) Antigenic demasking techniques in immunohistochemistry 8) Automation in immunohistochemistry 9) Principles of diagnostic immunohistochemistry 11) Immunohistochemical characterization of the principal breast cancer prognostic and predictive factors 12) Quality control and monitoring of the pre-analytic phase.
Course Language
Italian
More information
Integrazione a tutta l'attività didattica, con Seminari di interesse come:1) La sicurezza in laboratorio: la gestione del rischio.2) storia e uso dei coloranti in medicina e nell'alimentazione.3)La nascita dei vaccini: storia e pandemie del vecchio e nuovo secolo.4) conoscere gli strumenti ottici: uso e storia.
