Knowledge of the main techniques for measuring drug concentrations in biological matrices for pharmacological monitoring; study of some topics of pharmacokinetics and clinical pharmacology.
Course Prerequisites
To follow the course better, the student must have attended the lessons and acquired the knowledge in the basic subjects of the first semester.
Teaching Methods
Face-to-face lectures carried out through presentations (Power Point) projected on the screen and possible further information using the blackboard.
Assessment Methods
Oral exam carried out face-to-face. Graphics is emphasized: the student must know how to draw drug plasma concentration - time curves, etc.
Texts
M. Fuccella, E. Perucca, C. Sirtori - Farmacologia Clinica . UTET 2006. ISBN-10 : 8802053928 The lecturer will provide the presentations in pdf format or in another format and material of study of the topics discussed.
Contents
Pharmaceutical forms. Concept of solution, emulsion and suspension, isotony. Parenteral preparations: preparation of mixtures for parenteral feeding and for hydro-electrolytic rebalancing (sterility, apyrogenicity, isotony, pH, stability). ADME. Dose - drug blood level relationship, plasma concentration-time curves, therapeutic range. Utility of drug monitoring in biological fluids. Drugs for which monitoring is indicated and main methods used. Determination of the unbound fraction of drug and its usefulness. Main pharmacokinetic parameters and their determination: apparent volume of distribution, clearance, bioavailability, half-life and steady-state, elimination constant, bioequivalence. Compliance. Causes of variability in drug response. Notes on controlled clinical trial. Preparation of biological samples for the determination of drugs and their metabolites (liquid-liquid, liquid-solid extraction, etc.). Homogeneous phase (EMIT, FPIA) and heterogeneous phase (RIA, ELISA, MEIA) immunological methods. Chromatographic methods: High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). Construction of a calibration curve and qualitative and quantitative evaluation of the analytes contained in unknown biological samples. Notes on the validation of analytical methods: within study validation and practical applications. Quality control in the drug monitoring laboratory. Limits and potential of the usual analytical methods.
Course Language
Italian
More information
The lecturer receives by appointment face-to-face or via the internet (Zoom platform) and can be contacted by email at fausto.feletti@unipv.it.
