The course aims to provide the student with the cognitive and methodological tools necessary to: -correlate the composition, structure and physiology of pathogens with the evolution of the diseases they cause; -understand the role of different viruses as agents of human pathologies -understand the methodological approach in the diagnostic investigation of diseases caused by infection with viruses; -understand the epidemiology of infections. - acquire knowledge in the diagnostic field, both with regard to direct and indirect diagnosis of viral infections At the end of the course, the student should know: (i) structure and replicative cycle of viruses (ii) transmission routes of viruses and pathogenesis of viral infections; (iii) basic principles of direct and indirect virological diagnosis; (iv) knowledge of the main viral families to which human pathogenic viruses belong.
Course Prerequisites
The aim of the course is to provide students with the necessary tools to understand the relationships between different pathological conditions and potential etiological agents. To facilitate comprehension, students should have prior knowledge in basic subjects such as biochemistry, anatomy, physiology, immunology, and general pathology.
Teaching Methods
The course includes classroom lectures and discussions and presentations of case reports
Assessment Methods
EXAMINATION METHODS: WRITTEN TEST + ORAL TEST. Written test with markers: 25 quizzes on Virology/Clinical Microbiology, Bacteriology and Mycology (5 possible options of which 1 correct). Oral test only in the event of passing the written test, which will be considered passed with at least 80% of the correct answers. The overall assessment will derive both the written test and the oral test. In at the oral test, the result of the marked test will be shown (not before).
Texts
Bibliography for those attending lessons:
PDF of Slides available on the platform KIRO
P.R. Murray, K.S. Rosenthal, M.A. Pfaller Microbiologia Medica, VIII edizione Elsevier
J.C Sherris Microbiologia Medica, VI edizione EMSI Ed
G. Antonelli, M. Clementi, G. Pozzi, G.M. Rossolini Principi di Microbiologia Medica, III edizione Casa Editrice Ambrosiana
M. La Placa Principi di Microbiologia Medica, XIV edizione Esculapio, Bologna
Jawetz, Melnick & Adelberg’s Microbiologia Medica, XXVII edizione Piccin, Padova
Contents
Viruses: taxonomy, morphology, structure, chemical composition and methods of study - Virus-cell relationships and mechanisms of viral replication - Virus genetics - Culture of viruses and their titration - The mechanism of action and protective role of interferon and other cytokines in viral infections - Virus-host relationships; lytic and non-lytic infections - Cell transformation and oncogenesis - Antigenic properties of virions - Transmission of viruses and pathogenesis of viral infections - Virological diagnosis in human medicine - Classification and mechanism of action of antiviral drugs Systematics: DNA viruses: Papillomavirus, Poliomavirus, Herpesvirus, Adenovirus, Parvovirus, Poxvirus, Hepadnavirus. RNA viruses: Picornaviruses, Coronaviruses, Orthomyxoviruses, Paramyxoviruses, Pneumoviruses, Rabdoviruses, Filoviruses, Hepatitis viruses (A, C, D, E), Reoviruses and gastrointestinal viruses, Retroviruses (HTLV, HIV), viruses transmitted by arthropods and rodents: Bunyavirales, Arenaviridae, Togaviruses, Flaviviruses.