The laboratory experience aims to provide students with practical knowledge on the manipulation of microorganisms of medical interest and how to perform first- and second-level diagnostic tests. Special attention will be given to the interpretation of susceptibility tests and the critical interpretation of antibiograms. A general overview of diagnostic algorithms used in clinical microbiology laboratories will also be provided.
Course Prerequisites
The laboratories will be conducted toward the end of the Microbiology course and before the first scheduled exam. To best follow the lab, students must have attended the Microbiology course lectures and acquired the relevant knowledge.
Teaching Methods
Practical laboratory experiences conducted in equipped laboratories. The teaching materials will primarily consist of a handout prepared by the professor with protocols and examples of the tests performed, which will be sent to students after the practical activity.
Assessment Methods
No learning assessment is planned.
Texts
No textbook required.
Contents
- Bacterial culture in liquid and agar media, - Optical microscope observation of slides post-Gram stain - Preparation of susceptibility testing using the Kirby Bauer and E-test methods - Biochemical tests for presumptive identification of microorganisms (e.g., oxidase and catalase tests) - Preparation of phenotypic tests to confirm the production of resistance enzymes to various classes of antibiotics (primarily beta-lactams) - Simulation of diagnostic workflow from positive blood cultures - Reading and analysis of test results.
