Data di Pubblicazione:
2024
Abstract:
The study of Organic Residue Analysis (ORA) in archaeological pottery aims to detect traces of organic
materials, such as food and beverages, that were once contained in the vessels. Chemical analysis of or-
ganic residues can confirm hypotheses made by archaeologists regarding some important aspects of an-
cient daily life, from ancient diet to rituals. If non-invasive analytical techniques, such as spectroscopies,
could provide issues with overinterpretation of the data, chromatographic techniques combined with var-
ious detectors may be more suitable for separating and identifying the different components within a
complex matrix, like archaeological pottery. This research aims to develop a new rapid, reproducible
and efficient method for the identification of organic acids as wine markers in figured vessels t hrough
HPLC-MS/MS. The procedure included a derivatization step and an extraction step, both designed
based on green analytical chemistry principles. It employed ultrasound-assisted liquid extraction and dis-
persive liquid-liquid microextraction (dLLME). dLLME allowed to remove compounds that induce signal
suppression, thereby minimizing the matrix effect. Except for tartaric acid, which had a recovery around
20 %, the other analytes had recoveries that ranged from 40 % to 60 %, while LODs were comprised
be- tween 0.01 and 0.05 ng mL −1 . This method was applied to examine the potential presence of
wine in figured pottery, validating the method on historical samples in the frame of the wide-scope
project Imag-ORA
materials, such as food and beverages, that were once contained in the vessels. Chemical analysis of or-
ganic residues can confirm hypotheses made by archaeologists regarding some important aspects of an-
cient daily life, from ancient diet to rituals. If non-invasive analytical techniques, such as spectroscopies,
could provide issues with overinterpretation of the data, chromatographic techniques combined with var-
ious detectors may be more suitable for separating and identifying the different components within a
complex matrix, like archaeological pottery. This research aims to develop a new rapid, reproducible
and efficient method for the identification of organic acids as wine markers in figured vessels t hrough
HPLC-MS/MS. The procedure included a derivatization step and an extraction step, both designed
based on green analytical chemistry principles. It employed ultrasound-assisted liquid extraction and dis-
persive liquid-liquid microextraction (dLLME). dLLME allowed to remove compounds that induce signal
suppression, thereby minimizing the matrix effect. Except for tartaric acid, which had a recovery around
20 %, the other analytes had recoveries that ranged from 40 % to 60 %, while LODs were comprised
be- tween 0.01 and 0.05 ng mL −1 . This method was applied to examine the potential presence of
wine in figured pottery, validating the method on historical samples in the frame of the wide-scope
project Imag-ORA
Tipologia CRIS:
1.1 Articolo in rivista
Elenco autori:
Bartolini, F.; Vincenti, F.; Croce, M.; Ciccola, A.; Serafini, I.; Pola, A.; Favero, G.; Montesano, C.; Sergi, M.
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