Data di Pubblicazione:
2012
Abstract:
A purine nucleoside phosphorylase from Aeromonas hydrophila was cloned, expressed in Escherichia coli and purified. The enzyme showed a good stability
and a broad substrate specificity towards modified purine ribonucleosides. The enzyme was used for the synthesis of purine nucleosides structurally related
to compounds of biological interest via a “one pot, one enzyme” transglycosylation reaction using 7-methyl guanosine iodide as the sugar donor
and 6-substituted purines as acceptor.
and a broad substrate specificity towards modified purine ribonucleosides. The enzyme was used for the synthesis of purine nucleosides structurally related
to compounds of biological interest via a “one pot, one enzyme” transglycosylation reaction using 7-methyl guanosine iodide as the sugar donor
and 6-substituted purines as acceptor.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
nucleoside phosphorylase; Aeromonas hydrophila; 6-substituted purine ribonucleosides; enzymatic transglycosylation
Elenco autori:
Morelli, C. F.; Ubiali, Daniela; Serra, Immacolata; Biagiotti, M.; Borghese, Giovanni; Pappalardo, V. M.; Albertini, Alessandra; Speranza, G.
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