Novel ether-linkages containing aliphatic copolyesters of poly(butylene 1,4-cyclohexanedicarboxylate) as promising candidates for biomedical applications

A newclass of biodegradable and biocompatible poly(butylene 1,4-cyclohexanedicarboxylate) based randomcopolymers
are proposed for biomedical applications. The introduction of ether–oxygen containing BDG sequences
along the PBCE macromolecular chain is expected to remarkably improve chain flexibility and surface hydrophilicity
due to the presence of highly electronegative oxygen atoms.
P(BCExBDGy) copolymers were synthesized by polycondensation. The homopolymer PBCE and three copolymers,
namely (P(BCE70BDG30), P(BCE55BDG45) and P(BCE40BDG60)) were characterized from themolecular,
thermal, structural andmechanical point of view. Hydrolytic degradation studies in the presence and absence of
hog-pancreas lipase were performed under physiological conditions. To evaluate the diffusion profile of
small molecules through the polymer matrix, the release behaviour of fluorescein isothiocyanate (FITC) was
investigated. For biocompatibility studies, cell adhesion and proliferation of murine fibroblast (L929) and endocrine
pancreatic (INS-1) cells were performed on each polymeric film.
Results showed that solid-state properties can be tailored by simply varying copolymers' composition. Crystallinity
degree and hydrophobicity significantly decreasedwith the increase of BDGco-unitmol%.Moreover, mechanical
properties and biodegradability of PBCE, both depending on crystallinity degree,were remarkably improved:
P(BCE40BDG60) showed an elastomeric behaviour with εb over 600% and, as regard to biodegradability, after
98 days it lost over 60% of its initial weight if incubated in the presence of the pancreatic lipase. Lastly, the
newly developed biomaterials resulted not cytotoxic with both types of cells and could be properly tailored for
biomedical applications varying the content of BDG co-unit mol%.