Set up of methodological analysis to evaluate antioxidant capacity in serum and saliva using DPPH test method

Body oxygen consumption, during physical
activity, especially high intensity or due
to high stress environment, in the muscle fibers
engaged in the performance can increase
up to 200 times compared to that consumed
at resto In Football, it seems that the major
mechanisms responsible for oxidative stress
are: xanthine/Xanthine oxidase, eccentric
muscle damage with micro-inflammation,
leakage of electrons. Part of these oxygen
molecules, in mitochondria, can undergo reduction
with an increase in ROS production,
the latter can have different negative influences
on many cellular functions with both
acute and chronic mechanisms. Human body
has a complex defence system from oxidizing
agents, which acts synergistically promoting
ROS inactivation. These systems may not be
sufficient to cover oxidative stress that can
occur in strenuous exercise or during strenuous
athIetic seasons as a professional football
league. In this work, we explored the possibility
to monitor antioxidant capacity, with noninvasive
method, of some Italian professional
football players, during 2010/2011 season.
Methods. We have applied method developed
by Atsumi in 1999, which used 1,1'-diphenyl2-
picrylhydrazyl (DPPH radical), using human
samples, such as saliva and serum. We
have tested two different methods to treat
sample serum and we have compared serum
vs saliva results, the latter is considered a
diagnostic window on the blood and a valid
non invasive method.